How to make a CRISPR cow

How to make a CRISPR cow

Genome editing offers an opportunity to introduce useful genetic traits into livestock breeding programmes. However, it has proven difficult to insert large DNA fragments into livestock embryos using the CRISPR-Cas9 system. Dr Alison Van Eenennaam and Dr Joseph Owen, of the University of California, Davis, employed their knowledge of bovine embryogenesis and DNA repair pathways, and the help of a […]

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Directed evolution of CRISPR-Cas9 to increase specificity

Directed evolution of CRISPR-Cas9 to increase specificity

Building upon previous CRISPR research and gene editing methods found in nature, Dr Lee and colleagues from ToolGen have developed a method of screening multiple Cas9 variants. Their method seeks to quickly and effectively screen many mutations of Cas9 to find one which possesses maximised on-target activity and minimised off-target activity. After successful development and implementation of the system, Sniper-Screen, […]

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