Cell culture protocols for growing human keratinocytes in the laboratory

Dr Hitomi Fujisaki studies human epidermal keratinocytes (skin cells).

Dr Hitomi Fujisaki, Senior Research Scientist at Nippi Research Institute of Biomatrix in Japan, studies human epidermal keratinocytes (skin cells) and cancer cells using two- and three-dimensional type I collagen substrates. Through her research, she aims to understand more about the ways in which these cells interact with the substrate that they are grown on, and how this relationship can […]

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Uncovering critical cell death mechanisms for novel cardioprotective therapies

Mitochondria are required to produce the vast amount of energy that is required for the cell to beat.

Cell death is part of a natural process by which old cells are replaced by new, but it can also result from disease or injury. There are still many aspects of this process which are not fully understood. The laboratory of Dr Jason Karch, Baylor College of Medicine, aims to increase comprehension of the mechanisms behind cell death. If these […]

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Transtympanic injections to prevent hearing loss caused by cisplatin

Combination therapies with an otoprotectant may help to prevent hearing loss caused by chemotherapeutic agent cisplatin.

Dr Sofia Waissbluth from The Pontificia Universidad Católica de Chile is specialised in otolaryngology (conditions of the head and neck). She has reviewed clinical trials that use transtympanic injections to prevent hearing loss caused by the chemotherapeutic agent cisplatin. To date there is no ‘one-shot’ solution to protect or cure patients from hearing loss. However, combinational therapy using transtympanic injections […]

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Infectious bronchitis virus affects energy production

Infectious bronchitis virus affects energy production

Professor Tereza Cristina Cardoso, São Paulo State University, is a qualified veterinarian and her research explores infectious diseases in animals. Her latest work investigates how infectious bronchitis virus affects energy production by macrophage cells. Her research demonstrates that this occurs through disruption of mitochondrial respiration processes, which may have implications for how virus infections in poultry are prevented and treated. […]

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Stentor coeruleus: Do these tiny cells have out-of-body experiences?

Female patient has an out-of-body experience.

Occasionally, a person who recovers from a medical crisis reports an “out-of-body experience”. In unique research, Dr Benjamin Scherlag of the University of Oklahoma, Oklahoma City, USA, is using a single-celled organism called Stentor coeruleus to re-create the events that occur during an out-of-body experience. Dr Scherlag has shown that these cells can be revived from an apparently dead state […]

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Curiosity, cell death and caspases: One researcher’s journey to big discoveries

All comic book superheroes have an intriguing ‘origins story’; and in the case of science superman Professor Vishva Dixit he has one too. Beginning in Kenya and ending in California, this is the story of a researcher who asked big questions of himself and the world, and went on to pioneer a field of research into cell death, caspases and apoptosis. […]

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Fluorescent imaging sheds new light on apoptosis

Fluorescent imaging sheds new light on apoptosis

Researchers at the Avera Cancer Institute are using state-of-the-art fluorescent imaging to shed new light on the physical and biochemical processes behind apoptosis – also known as ‘programmed cell death’. The novel, cost-effective and user-friendly method uses three fluorescent stains, enabling the real-time, structure-function based identification of apoptosis in live, apoptotic tumour cells. This unique protocol developed by Dr Nandini […]

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